What additional precautions are taken at BSL-2 compared to BSL-1?

At BSL-2, additional precautions such as restricted access and the use of personal protective equipment (PPE) are implemented to ensure a higher level of safety for laboratory personnel when handling moderately hazardous biological agents. This tier of biosafety is designated for work with organisms that may pose a risk through ingestion, inhalation, or puncture.

The requirement for restricted access minimizes the chances of unauthorized individuals entering the laboratory, which is crucial when working with pathogens that can cause mild or moderate diseases in humans. Furthermore, the mandatory use of PPE, including gloves, gowns, and eye protection, is essential for personal safety and helps to prevent the spread of potentially infectious materials.

Additional differences from BSL-1, which typically involves low-risk organisms that pose minimal threat to laboratory workers and the environment, include specific protocols for handling infectious materials safely, waste decontamination procedures, and the introduction of safety equipment like biosafety cabinets. These enhancements underscore the importance of maintaining a controlled environment to protect laboratory personnel and public health.