Which of the following is a critical operation of a BSL-2 laboratory?

In a BSL-2 laboratory, the use of personal protective equipment (PPE) is a critical operation because it helps ensure the safety of personnel working with biological agents that pose moderate hazards. PPE, such as gloves, lab coats, and eye protection, is designed to minimize exposure to potentially infectious materials, preventing contamination of the laboratory worker and their environment. This practice is essential in maintaining a barrier against contamination when handling specimens that could contain pathogenic microorganisms.

Effective use of PPE significantly reduces the risk of transmission of these agents, thus protecting both the individual and public health. By mandating the proper use of PPE, BSL-2 laboratories adhere to biosafety standards that aim to lessen the risk of exposure and spread of infectious diseases.